Tools
NAD+ reconstitution calculator
By An NayalLast reviewed 1 source
NAD+ comes in large vials - 500 or 1000 mg - so the milligram-to-units conversion is easy to get wrong, and clinics often dose the made-up solution by volume instead of by mg. Enter your vial and Pepteca does the math, including how many doses the vial holds.
Draw this much
50units
= 0.5 mL at 100 mg/mL
This vial holds about 20 full doses at this size.
Math conversion only. This does not tell you what dose to take. Always verify with a licensed provider.
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Worked example
The calculator opens on a 1000 mg vial, 10 mL of bacteriostatic water, a 50 mg dose and a 1 mL syringe. Here is what it does with those numbers.
- Concentration: 1000 mg / 10 mL = 100 mg/mL.
- Volume: 50 mg / 100 mg/mL = 0.5 mL.
- Units: 0.5 mL x 100 = 50 units.
- Doses per vial: 1000 mg / 50 mg = 20 full doses.
The result card reads 50 units, “= 0.5 mL at 100 mg/mL”, and says the vial holds about 20 full doses. Half a millilitre fills half of the 1 mL barrel, so there is no warning. Pick the 0.3 mL syringe instead and the same draw is over capacity, because 0.5 mL is more than it holds.
A 500 mg vial works the same way. Mixed with 5 mL of water it is also 100 mg/mL, so 50 mg is still 50 units, but the vial now holds 10 full doses instead of 20.
How the math works
concentration (mg/mL) = vial mg / water mL
draw (mL) = dose mg / concentration
draw (units) = draw mL x 100
doses per vial = vial mg / dose mg, rounded down
Reconstitution is one division. The concentration is the vial mass divided by the water you add. The volume for a dose is the dose divided by that concentration, and the units are that volume times 100, because a U-100 insulin syringe carries 100 units to the mL. Doses per vial is the vial mass divided by the dose, rounded down, since the leftover under one full dose is not usable. If you switch the dose to mcg, it is divided by 1000 first. These are exact unit conversions; they do not tell you what dose to take.
One NAD+ wrinkle: because it is a compounded product with no manufacturer standard, subcutaneous protocols are often written in millilitres of a made-up solution rather than in milligrams. Knowing your mg/mL is what lets you translate a “0.2 mL” instruction back into an actual dose.
Sources
- [1]Empower Pharmacy - NAD+ Injection (compounding pharmacy product page)communityNAD+ injection is a 503A-compounded product supplied as a pre-mix or a powder to reconstitute; vial sizes and mixing ratios vary because there is no FDA-approved manufacturer standard.
The full evidence base and citations for NAD+ live on the injection reference page.
Questions people ask
How many units is 50 mg of NAD+?
It depends on the mix. A 1000 mg vial with 10 mL of bacteriostatic water is 100 mg/mL, so 50 mg is 0.5 mL, which is 50 units on a U-100 insulin syringe. The same vial with 5 mL of water is 200 mg/mL, so 50 mg is 0.25 mL, or 25 units.
How many doses are in a 1000 mg NAD+ vial?
The calculator divides the vial mg by the dose mg and rounds down, because a part-dose left at the bottom is not a full dose. At 50 mg that is 1000 / 50 = 20. At 100 mg it is 10. Real vials lose a little to dead space in the syringe, so expect slightly fewer.
My protocol is written in mL, not mg. How do I convert it?
Multiply the volume by your concentration. At 100 mg/mL, 0.2 mL is 20 mg. The calculator shows your mg/mL under the result, so you can do that step for your own vial.
What if the draw is more than 100 units?
A draw over 1 mL does not fit a 1 mL insulin syringe, and the calculator flags it as over capacity. The same check applies to the 0.3 mL and 0.5 mL syringes at 30 and 50 units.
Pepteca is for education and information only. It does not provide medical advice, diagnosis, or treatment, and its calculators perform unit math only. Always verify with a licensed provider before starting, changing, or stopping any protocol. Many peptides are not approved for human use and may be regulated differently where you live.